Location of unique sequences in tobacco mosaic virus ribonucleic acid.

Tobacco mosaic virus has been partially stripped of its protein coat by sodium dodecyl sulfate. The nucleic acid exposed was digested with T1 ribonuclease and the products were examined for the presence of three oligonucleotides of unique sequence. The results indicate the location of two of the unique oligomers to be near the 5’-OH-linked end and the third to be near the middle of tobacco mosaic virus RNA. They also provide evidence that the cistron for tobacco mosaic virus coat protein is not situated at the 5’OH-linked terminus.